Cyclotide-rich fractions containing nanofibers by electrospinning: preparation, characterization and examination of antimicrobial activity

In this study, antimicrobial nanofibers were produced with the mixtures of polyvinyl alcohol (PVA) and cyclotide-rich fractions by electrospinning method. After extraction, first separation was carried out C18 flash chromatography then fractioned into five separate parts reversed-phase high-pressure liquid (RP-HPLC). The molecular weights cyclotides in each fraction determined quadrupole time-of-flight chromatography-mass spectrometry (Q-TOF LC-MS). Cyclotide-rich mixed 10% PVA solution from biocomposite mixture characterized field emission scanning electron microscopy (FE-SEM), it observed that 100% peptide-containing (cyclotide-rich fraction/10% PVA, w/v) had more regular fiber textures. presence peptides nanofiber also confirmed analytical RP-HPLC, as both peptide solutions have same retention times. fourth showed activity against gram-positive bacteria (Bacillus cereus) susceptibility test. As a result these findings, cyclotide-containing can be for pharmaceutical research development studies.